本教材编写中着力体现如下特色：1. 形态学观察，体现数码互动实验室等新技术新手段；2. 接种培养，展望细菌自动接种、基于生化反应的自动鉴定系统和自动药敏系统；3. 病原的分子检测，体现或展望基于抗原抗体检测的自动化流水线，基于代谢产物检测的质谱；以及基于核酸检测的荧光定量（RT）或数字PCR等；4. 验证实验体现科学性和体系性，综合实验体现探索性和创新性；5. 教育理念，体现标本采集的科学性与伦理性、实验过程及废弃物处理的生物安全、动物实验的动物伦理等课程思政和生物安全元素。

本书分为三篇共三十七个实验。第一篇为免疫学基础实验，包括血清总补体溶血活性（CH50）的测定等七个实验；第二篇为病原生物学基础实验，包括细菌的接种、培养与生化反应等二十二个实验；第三篇为综合性实验，包括多克隆抗体的制备及效价测定等七个实验。书后还有附录和部分实验结果的彩图。所有内容均用中文和英文两种语言编写。本书不仅可作为高等医学院校临床医学、医学检验技术、中西医临床医学等专业的病原生物学与免疫学实验教材，还可作为医院、卫生防疫部门中从事病原生物学与免疫学工作的技术人员的参考书。

第一篇免疫学基础实验/1实验一血清总补体溶血活性（CH50）的测定/1实验二人外周血单个核细胞的分离/3实验三E花环形成试验/5实验四中性粒细胞吞噬功能测定/7实验五双向琼脂扩散试验/8实验六PCRSSP法检测HLAA*02基因/9实验七利用SPA亲和层析法分离纯化IgG抗体/11实验八人血清总IgE检测/12第二篇病原生物学基础实验/14实验一细菌的生理/14实验二细菌分布及外界因素对细菌的影响/19实验三细菌形态结构的检查/22实验四化脓性球菌/24实验五肠道杆菌/26实验六厌氧性细菌/30实验七分枝杆菌/31实验八其他细菌/32实验九病毒/34实验十真菌/35实验十一内毒素检测（鲎试验）/37实验十二寄生关系及寄生虫演化/40实验十三吸虫/41实验十四绦虫/44实验十五线虫/46实验十六常见蠕虫的检验方法/49实验十七阿米巴/55实验十八孢子虫/57实验十九鞭毛虫/60实验二十纤毛虫/62实验二十一原虫检查方法/62实验二十二医学节肢动物/65第三篇综合性实验/71实验一多克隆抗体的制备及效价测定/71实验二流式细胞术检测小鼠脾脏T、B细胞的比例/73实验三呼吸道感染常见病原微生物的实验室检查/74实验四肠道杆菌的分离鉴定/78实验五泌尿生殖道感染常见病原微生物的实验室检查/82实验六皮肤创伤感染常见细菌的实验室检查/84实验七日本血吸虫感染小鼠致病及免疫学诊断/86ContentsChapter 1Basic Experiments of Immunology/88Experiment 1Measure of the 50% Haemolytic Complement （CH50） Activity of Serum/88Experiment 2Isolation of Human Peripheral Blood Mononuclear Cells/91Experiment 3Erosette Forming Test/92Experiment 4Neutrophil Phagocytosis Test/95Experiment 5Double Immunodiffusion Test/97Experiment 6Detect the HLAA*02 Gene by PCRSSP/98Experiment 7Purify IgG Antibody by SPA Affinity Chromatography/100Experiment 8Detection of Total IgE in Human Serum/102Chapter 2Basic Experiments of Pathogenic Biology/104Experiment 1Physiology of Bacteria/104Experiment 2The Distribution of Bacteria and the Effect of External Factors on Bacteria/112Experiment 3Examination of Bacterial Morphology and Structure/116Experiment 4Pyogenic Coccus/118Experiment 5Enterobacteriaceae/122Experiment 6Anaerobic bacteria/126Experiment 7Mycobacterium/127Experiment 8Other bacteria/129Experiment 9Virus/132Experiment 10Fungi/134Experiment 11Test of Endotoxin （Limulus Amoebocyte Lysate Test）/136Experiment 12Parasitic Relationships and Parasite Evolution/139Experiment 13Trematode/140Experiment 14Tapeworm/146Experiment 15Nematode/148Experiment 16Examination of Helminths/152Experiment 17Amoeba/160Experiment 18Sporozoa/163Experiment 19Flagellates/166Experiment 20Ciliates/168Experiment 21Methods for Protozoa Diagnosis/169Experiment 22Medical Arthropods/173Chapter 3Comprehensive Experiment/175Experiment 1Preparation and Titer Determination of Polyclonal Antibody/175Experiment 2Measure the Ratio of T and B Cells in the Spleen by Flow Cytometry/177Experiment 3Laboratory Examination of Common Pathogenic Microorganism of Respiratory Tract Infection/179Experiment 4Isolation and Identification of Enterobacteriaceae/184Experiment 5Laboratory Examination of Common Pathogens of Genitourinary Tract Infection/190Experiment 6Laboratory Examination of Common Bacteria in Skin Wound Infections/193Experiment 7Pathogenic and Immunological Diagnosis of Mice Infected with Schistosoma japonicum/196附录A常用染色液和试剂的配制/199附录B常用培养基的配制/201Appendix APreparation of Common Staining Solution and Reagents/203Appendix BPreparation of Common Culture Medium/205部分实验结果的彩图/208Color Figures of Some Experimental Results/208参考文献/225