本书内容分为三大部分：生物化学实验概论（PartⅠ）、专项实验（PartⅡ~PartⅥ）、附录。第一部分包括生物化学实验的基本要求、基本技能及生物化学技术的基本原理（Chapter1~6）。根据系统的特点，本书从蛋白质实验、酶学实验、糖脂实验、核酸实验、设计性实验、生物信息学实验等方面进行了专题实验，共35个实验。每一个实验中，在简要介绍了实验原理后，重点阐述了实验过程中的要点、技术分析和注意事项。最后，分析了可能遇到的问题、潜在原因及解决办法。

Part Ⅰ General Introduction to Biochemistry Experiment
Chapter 1 Basic Requirements ofBiochemistry Experiment
Section 1 Basic Laboratory Requirements
1.1 Biochemistry laboratory rules
1.2 Biochemistry laboratory safety
Section 2 Experimental Record and Data Processing
2.1 Experimental record
2.2 Data processing
2.3 Requirements of the laboratory report
Chapter2 Basic Skills in Biochemistry Experiment
Section 1 Mixing of Solutions
1.1 Vibration mixing
1.2 Mixing solutions with instruments
Section 2 The Use of.Graduated Pipettes
2.1 Types of the graduated pipettes
2.2 The procedure of using graduated pipettes
Section 3 The Use of Afljustable Micropipettes
3.1 The procedure of using adjustable micropipette
3.2 Notes
Chapter3 Spectrophotometry
Section 1 Basic Principles
1.1 Lambert—Beer’S law
1.2 The application of Lambert——Beer's law
Section 2 The Basic Construction of Spe《：trophotonleters and It's Usage
2.1 The basic construction of spectrophotometers
2.2 Usage of the typical spectmphotometer
2.3 Notes
Chtapter 4 Electrophoresis
Section 1 Basic Principle of Electro[}h(，resisⅥ
1.1 Generation of charged particles
……
Part Ⅱ Protein Experments
Part Ⅲ Enzymology Experiments
Part Ⅳ Carbohydrate and Lipid Experiments
Part Ⅴ Nucleic Acid Experiments
Part Ⅵ Design of Experiment and Informational Experiment